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- domestic rabbit monoclonal (JE38-80)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of PAF1 on different lysates with Rabbit anti-PAF1 antibody (HA722401) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722401) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Paf1
- synonym:RGD1306219
- description:PAF1 homolog, Paf1/RNA polymerase II complex component
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product type
- Paf1 antibody
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