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- domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot
citations: 3
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-P2X6 Receptor (extracellular) Antibody (#APR-028), (1:200).3,4. Anti-P2X6 Receptor (extracellular) Antibody, preincubated with P2X6 Receptor (extracellular) Blocking Peptide (#BLP-PR028).
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2 
Western blot analysis of rat brain membranes: - 1. Anti-P2X6 Receptor Antibody (#APR-013), (1:200). 2. Anti-P2X6 Receptor Antibody, preincubated with P2X6 Receptor Blocking Peptide (#BLP-PR013).
Expression of P2X6 in rat hypothalamus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-P2X6 Receptor Antibody (#APR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2X6 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with P2X6 Blocking Peptide (BLP-PR013), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.
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to the supplier - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of P2X6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: K562 cell lysate
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-P2X6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2X6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - rat P2rx6
- synonym:P2rxl1; P2x6; Rnap2x6
- description:purinergic receptor P2X 6
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- P2rx6 antibody
- P2rx6 protein
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