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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: Caco-2 cell lysate Lane 4: K-562 cell lysate Lane 5: A431 cell lysate Lane 6: L-929 cell lysate Lane 7: MEF cell lysate Lane 8: PC-12 cell lysate Lane 9: Human kidney tissue lysate Lane 10: Mouse kidney tissue lysate Lane 11: Mouse heart tissue lysate Lane 12: Mouse spleen tissue lysate Lane 13: Rat kidney tissue lysate Lane 14: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500544) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (PSH05-07)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA722223) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Caco-2 cell lysate Lane 3: K-562 cell lysate Lane 4: A431 cell lysate Lane 5: MEF cell lysate Lane 6: PC-12 cell lysate Lane 7: Human kidney tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH05-07)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA750980) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Caco-2 cell lysate Lane 3: K-562 cell lysate Lane 4: A431 cell lysate Lane 5: MEF cell lysate Lane 6: PC-12 cell lysate Lane 7: Human kidney tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750980) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
gene information - rat Nsun5
- synonym:RGD1309268
- description:NOP2/Sun RNA methyltransferase 5
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Nsun5 antibody
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