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- domestic goat polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1 - domestic rabbit monoclonal (JE61-56)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of NPY5R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720061, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: A549 cell lysate Lane 4: Mouse spleen tissue lysate
ICC staining of NPY5R in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720061, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of NPY5R in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720061, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunoprecipitation, enzyme immunoassay - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot, immunohistochemistry, immunocytochemistry - domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of rat brain membrane: - 1. Anti-NPY5R Antibody (#ANR-025), (1:200).2. Anti-NPY5R Antibody, preincubated with NPY5R Blocking Peptide (#BLP-NR025).
Expression of neuropeptide Y5 receptor in rat striatum - Immunohistochemical staining of rat striatum using Anti-NPY5R Antibody (#ANR-025). A. NPY5R (red) appears in the striatal matrix (asterisk) and in nerve cells in the matrix (vertical arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Merge of NPY5R and parvalbumin demonstrates co-localization in a sub-set of cells (horizontal arrows). DAPI is used as the counterstain (blue).
Expression of neuropeptide Y5 receptor in rat DRG - Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY5R Antibody(#ANR-025) (1:100) followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. B E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images ofpanels D and E.Magnification:A-C: x20E-F: x100
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gene information - rat Npy5r
- synonym:NPYR5
- description:neuropeptide Y receptor Y5
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Npy5r antibody
- Npy5r protein
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