rat Nov antibody | 1 product cited in the literature; 6 total from 6 suppliers

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CCN3
Proteintech Group
catalog: 55449-1-AP

rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry
citations: 1

The NOV antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human NOV. This antibody recognizes human,mouse,rat antigen. The NOV antibody has been validated for the following applications: ELISA, WB, IHC analysis.
quantity: 150 ul
price: 299 USD
to the supplier
NOV Polyclonal Antibody
Invitrogen
catalog: PA5-79750

rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100µg
price: US 305
to the supplier
Anti-CCN3 [EPR8781(3)] antibody
Abcam
catalog: ab191425

rabbit monoclonal (EPR8781(3))
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

quantity: 10 µl, 100 µl
price:
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CCN3 / NOV Antibody (aa156-351) LS-C374871
LifeSpan Biosciences
catalog: LS-C374871

rabbit polyclonal
reactivity: human, rat
application: western blot

Western Blot; Sample: Rat Serum; Primary Ab: 2µg/ml Rabbit Anti-Rat NOV Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

quantity:
price:
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Anti-NOV/CCN3 Picoband Antibody
Boster
catalog: A06319-1

rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry

Figure 1. Western blot analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOV/CCN3 antigen affinity purified polyclonal antibody (Catalog # A06319-1) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOV/CCN3 at approximately 47KD. The expected band size for NOV/CCN3 is at 39KD.

Figure 2. IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1). . NOV/CCN3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1). . NOV/CCN3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. .
quantity: 100 ug/vial
price: 280 USD
to the supplier
CCN3 Antibody
Cell Signaling Technology
catalog: 8767

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity:
price:
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