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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody, preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).
Expression of Bombesin receptor 1 in rat hypothalamus - Immunohistochemical staining of frozen rat hypothalamus sections using Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:200). A. Staining (red) appears in the neuropil near the ventricle (asterisk) and in neurons (triangles). Calbindin D28k staining (green) appears in neurons. C. Merge of (A) and (B) shows co-expression of BB1R and Calbindin in a few neurons (vertical arrow). DAPI is used as the counterstain.
Western blot analysis of human lung carcinoma NCI-H526 (lanes 1 and 3) and human prostate carcinoma PC-3 (lanes 2 and 4) cell line lysates: - 12.Anti-Bombesin Receptor 1(NMBR)Antibody (#ABR-004) (1:1000).34. Anti-Bombesin Receptor 1 (NMBR) Antibody preincubated with the control peptide antigen.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of NMBR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500253, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse testis tissue lysate
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NMBR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500253, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-NMBR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500253, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Nmbr
- synonym:NMB-R
- description:neuromedin B receptor
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product type
- Nmbr antibody
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