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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200).3,4. Anti-NETO2 (extracellular) Antibody, preincubated with NETO2 (extracellular) Blocking Peptide (#BLP-GC046).
Expression of NETO2 in mouse and rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NETO2 immunoreactivity (green) in both mouse (A.) and rat (B.) cerebellum region is detected in the granule layer (G) and in “baskets” (arrows). Cell nuclei are stained with DAPI (blue).
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to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section 
Overlay histogram showing Jurkat cells stained with MBS9211489 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9211489, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Anti-NETO2 Antibody (N-term ) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
NETO2 Antibody (N-term ) (Cat. #MBS9211489)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NETO2 Antibody (N-term ) for immunohistochemistry. Clinical relevance has not been evaluated.
quantity: 0.08 mL
price:
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot
gene information - rat Neto2
- description:neuropilin and tolloid like 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Neto2 antibody
- Neto2 protein
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