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- domestic rabbit monoclonal (JE77-56)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of NELF-B on different lysates with Rabbit anti-NELF-B antibody (HA722320) at 1/1,000 dilution. Lane 1: T-47D cell lysate Lane 2: HeLa cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: C2C12 cell lysate Lane 10: C6 cell lysate Lane 11: L6 cell lysate Lane 12: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: Lane 1-7: 2 minutes; Lane 8-12: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722320) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (F7E4)
reactivity: human, mouse, rat
application: western blot, ELISA 
Western Blot of Rabbit Anti-COBRA1 Antibody. Lane 1: neonatal rat brain lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: COBRA1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~ 62kDa/~62kDa for COBRA1 protein. Other band(s): none.
quantity: 100 µL
price:
to the supplier - domestic rabbit polyclonal (2H4E9)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - rat Nelfb
- synonym:Cobra1; RGD1307832
- description:negative elongation factor complex member B
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Nelfb antibody
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