Your Filters
product
- domestic rabbit monoclonal (JE35-58)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (HA721191) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721191) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (HA721191) at 1/500 dilution. Lane 1: Rat skin tissue lysate Lane 2: Human skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721191) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA, enzyme immunoassay - domestic rabbit monoclonal (D82F4)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
gene information - rat Nedd4
- synonym:Nedd4a
- description:NEDD4 E3 ubiquitin protein ligase
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Nedd4 antibody
species
questions and comments