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- domestic rabbit monoclonal (JE55-38)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NAT10 on different lysates with Rabbit anti-NAT10 antibody (ET7111-23) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lane 3: Mouse stomach tissue lysate Lane 4: Rat colon tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 116 kDa Observed band size: 116 kDa Exposure time: Lane 1-2: 8 seconds; Lane 3-5: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-23) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NAT10 on different lysates with Rabbit anti-NAT10 antibody (ET7111-23) at 1/500 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si NAT10 cell lysate (10 µg/Lane) Predicted band size: 116 kDa Observed band size: 116 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. ET7111-23 was shown to specifically react with NAT10 in Hela-si NT cells. Weakened band was observed when Hela-si NAT10 sample was tested. Hela-si NT and Hela-si NAT10 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7111-23, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Daudi cells labeling NAT10 with Rabbit anti-NAT10 antibody (ET7111-23) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NAT10 antibody (ET7111-23) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (5Z3E5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
gene information - rat Nat10
- synonym:RGD1306717
- description:N-acetyltransferase 10
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- Nat10 antibody
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