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- domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 10 - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE65-01)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MYO1B KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/5,000 dilution. Lane 1: C6 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: Lane 1: 10 seconds; Lane 2-3: 2 minutes 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Myo1b
- synonym:Myr1
- description:myosin Ib
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product type
- Myo1b antibody
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