TPOR
HUABIO
catalog: HA722068

domestic rabbit monoclonal (JE58-00)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of TPOR on different lysates with Rabbit anti-TPOR antibody (HA722068) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of A431 cells labeling TPOR. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722068, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier