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- mouse monoclonal (36665)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, dot blot
citations: 39
Detection of Human MMP-1 antibody by Western Blot.
quantity: 200 ug (also 25 ug)
price: 489 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Raji cell lysate (15 µg/Lane) Lane 4: A431 cell lysate (15 µg/Lane) Lane 5: HeLa cell lysate (15 µg/Lane) Lane 6: HUVEC cell lysate (15 µg/Lane) Lane 7: A549 cell lysate lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lane 3: Raji cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP1 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Mmp1
- synonym:Clgn; MMP-1; Mmp1a
- description:matrix metallopeptidase 1
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product type
- Mmp1 antibody
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