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- Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] - BSA and Azide free
Abcam
catalog: ab248071domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry - domestic rabbit monoclonal (JG39-79)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Methionine Aminopeptidase 2 / p67 on different lysates with Rabbit anti-Methionine Aminopeptidase 2 / p67 antibody (ET7108-97) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: K-562 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 67 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Methionine Aminopeptidase 2 / p67 on different lysates with Rabbit anti-Methionine Aminopeptidase 2 / p67 antibody (ET7108-97) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Methionine Aminopeptidase 2 / p67 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 67 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-97) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of K-562 cells labeling Methionine Aminopeptidase 2 / p67 with Rabbit anti-Methionine Aminopeptidase 2 / p67 antibody (ET7108-97) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Methionine Aminopeptidase 2 / p67 antibody (ET7108-97) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - Phospho-MAP2 (Thr1620/1623) Antibody
Cell Signaling Technology
catalog: 4544
total number of products: 2domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry - paraffin section
citations: 1
gene information - rat Metap2
- synonym:Amp2; Mnpep; p67
- description:methionyl aminopeptidase 2
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product type
- Metap2 antibody
- Metap2 protein
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