MDH1 Antibody (JE35-03)
Novus Biologicals
catalog: NBP3-32582

domestic rabbit monoclonal (JE35-03)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 ul
price: 449 USD
to the supplier

MDH1
HUABIO
catalog: HA722196

domestic rabbit monoclonal (JE35-03)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of MDH1 on different lysates with Rabbit anti-MDH1 antibody (HA722196) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: U-937 cell lysate (20 µg/Lane) Lane 4: HL-60 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722196) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of U-937 cells labeling MDH1 with Rabbit anti-MDH1 antibody (HA722196) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MDH1 antibody (HA722196) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of U-937 cells labeling MDH1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722196, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

Anti-MDH1 antibody
Abcam
catalog: ab175455

domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 200µL
price:
to the supplier

CLEC5A / MDL1 / MDL-1 Antibody, Rabbit MAb
Sino Biological
catalog: 80259-R005

domestic rabbit monoclonal (005)
reactivity: rat
application: ELISA

quantity: 100 µL
price: 269 USD
to the supplier