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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
Western blot analysis of rat brain (lanes 1 and 4), kidney (lanes 2 and 5) and pancreas (lanes 3 and 6): - 1,2,3. Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083), (1:200).4,5,6. Anti-TRPML3 (Mucolipin 3) Antibody, preincubated with TRPML3/Mucolipin 3 Blocking Peptide (#BLP-CC083).
Expression of Mucolipin 3 in mouse brain stem - Immunohistochemical staining of TRPML3 in mouse brain stem using Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083). A. TRPML3 (green) appears in neurons (arrows) in the area of the dorsal cochlear nucleus. B. Staining of the same section with mouse anti-parvalbumin (red) reveals that the TRPML3 appears in a subset of neurons in this nucleus. DAPI is used as the counterstain (blue).
Peptide CKDLPNSGKYRLEDD corresponding to amino acid residues 528-542 of mouse TRPML3 (Accession Q8R4F0). Intracellular C-terminus (cytoplasmic).
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: ATTO 594
application: immunohistochemistry, immunocytochemistry 
Expression of Mucolipin 3 in mouse brain - Immunohistochemical staining of TRPML3 in mouse brain frozen section with Anti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody (#ACC-083-AR). A. Staining revealed TRPML3 positive nerve cell profiles in the brainstem auditory nucleus. B. Preincubation with the immunogen peptide suppressed the above staining pattern.
Expression of Mucolipin 3 in mouse brain - Immunohistochemical staining of TRPML3 in mouse brain frozen section with Anti-TRPML3 (Mucolipin 3)-ATTO-594 Antibody(#ACC-083-AR). A. Staining revealed TRPML3 positive nerve cell profiles in the brainstem auditory nucleus. B. Preincubation with the immunogen peptide suppressed the above staining pattern.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of TRPML3 on different lysates with Rabbit anti-TRPML3 antibody (ER1901-21) at 1/2,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: HL-60 cell lysate (10 µg/Lane) Lane 3: HEK-293 cell lysate (10 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Lane 7: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TRPML3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of TRPML3 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-21, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
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gene information - rat Mcoln3
- description:mucolipin 3
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product type
- Mcoln3 antibody
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