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- domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry
citations: 2
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-MC5 Receptor Antibody (#AMR-025), (1:200).3,4. Anti-MC5 Receptor Antibody, preincubated with MC5 Receptor Blocking Peptide (#BLP-MR025).
Expression of MC5R in rat skin - Immunohistochemical staining of paraffin embedded rat skin section using Anti-MC5 Receptor Antibody (#AMR-025), (1:100). MC5R staining is present in sebaceous glands. Hematoxilin is used as the counterstain.
Expression of MC5R in rat skin - Immunohistochemical staining of paraffin embedded rat skin section using Anti-MC5 Receptor Antibody(#AMR-025) (1:100). MC5R staining is present in sebaceous glands. Hematoxilin is used as the counterstain.
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to the supplier - domestic rabbit monoclonal (JE61-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of MC-2 on different lysates with Rabbit anti-MC-2 antibody (HA720055) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: MG-63 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Mouse hippocampus tissue lysate Lane 5: Rat hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 48 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720055) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MC-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720055, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Mc5r
- description:melanocortin 5 receptor
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product type
- Mc5r antibody
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