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- domestic rabbit monoclonal (JU38-30)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of EB3 on different lysates with Rabbit anti-EB3 antibody (ET7106-99) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: MG-63 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-99) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
EB3 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with ET7106-99 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7106-99 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input) Lane 2: ET7106-99 IP in U-87 MG cell lysate Lane 3: Rabbit IgG instead of ET7106-99 in U-87 MG cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot 
Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.
quantity: 0.1 mg (also 0.025 mg)
price: 329 USD
to the supplier - domestic rabbit monoclonal (7J9V3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
gene information - rat Mapre3
- description:microtubule-associated protein, RP/EB family, member 3
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Mapre3 antibody
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