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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal (PSH14-06)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (HA723596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: Jurkat cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: MEF cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 52/66 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
PRAK / MK5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723596 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723596 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723596 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723596 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1802
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH14-06)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation 
Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (HA751501) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: Jurkat cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: MEF cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 52/66 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751501) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
PRAK / MK5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751501 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751501 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751501 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751501 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1802
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (D70A10)
reactivity: human, rat
application: western blot
citations: 1
gene information - rat Mapkapk5
- description:MAPK activated protein kinase 5
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- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
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product type
- Mapkapk5 antibody
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