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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation - domestic rabbit monoclonal ([HHC-13])
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS
Western blot analysis of MEKK3 expression in Hela cell lysate (MBS179973). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K3 monoclonal antibody (MBS179973) overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K3
quantity: 0.03 mg
price:
to the supplier - domestic rabbit monoclonal (D36G5)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
citations: 2
gene information - rat Map3k3
- description:mitogen activated protein kinase kinase kinase 3
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product type
- Map3k3 antibody
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