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- mouse monoclonal (OTI3A10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
Western Blot: Lipoprotein Lipase/LPL Antibody (OTI3A10) - Azide and BSA Free [NBP2-71178] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoprotein lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lip
quantity: 100 ug
price: 579 USD
to the supplier - mouse monoclonal (OTI3A10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, FACS 
Overlay histogram showing Hela cells stained with MBS9207576 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207576, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-LPL Antibody (Center) at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
LPL Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LPL antibody detected the LPL protein (arrow).
quantity: 0.08 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: immunohistochemistry - paraffin section
gene information - rat Lpl
- description:lipoprotein lipase
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- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Lpl antibody
- Lpl ELISA/assay
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