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- domestic rabbit monoclonal (JU32-53)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]
quantity: 100 ul
price: 459 USD
to the supplier - domestic rabbit monoclonal (2.00E+05)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of LOXL2 on A549 and A431 cell lysate using anti-LOXL2 antibody at 1/500 dilution.
ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining LOXL2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (JU32-53)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of LOXL2 on different lysates with Rabbit anti-LOXL2 antibody (ET1706-11) at 1/500 dilution. Lane 1: PC-12 cell lysate, 10 µg/Lane Lane 2: A549 cell lysate, 10 µg/Lane Predicted band size: 87 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-11) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A431 cells labeling LOXL2 with Rabbit anti-LOXL2 antibody (ET1706-11) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LOXL2 antibody (ET1706-11) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of A549 cells labeling LOXL2 with Rabbit anti-LOXL2 antibody (ET1706-11) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LOXL2 antibody (ET1706-11) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Loxl2
- description:lysyl oxidase-like 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Loxl2 antibody
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