Your Filters
product
- domestic rabbit monoclonal (PSH08-82)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of LIMK2 on different lysates with Rabbit anti-LIMK2 antibody (HA723034) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SW480 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: F9 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 72 kDa Observed band size: 70 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723034) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LIMK2 on different lysates with Rabbit anti-LIMK2 antibody (HA723034) at 1/2,000 dilution. Lane 1: Human LIMK2 recombinant protein, 20ng/Lane Lane 2: Human LIMK1 recombinant protein, 20ng/Lane Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723034) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Ranos cells labeling LIMK2 with Rabbit anti-LIMK2 antibody (HA723034) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMK2 antibody (HA723034) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH08-82)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of LIMK2 on different lysates with Rabbit anti-LIMK2 antibody (HA751247) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SW480 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: F9 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 72 kDa Observed band size: 70 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751247) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LIMK2 on different lysates with Rabbit anti-LIMK2 antibody (HA751247) at 1/2,000 dilution. Lane 1: Human LIMK2 recombinant protein, 20ng/Lane Lane 2: Human LIMK1 recombinant protein, 20ng/Lane Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751247) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Ranos cells labeling LIMK2 with Rabbit anti-LIMK2 antibody (HA751247) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMK2 antibody (HA751247) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal (JB80-36)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section 
Western Blot: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Analysis of extracts from Hela cells untreated or treated with PMA using LIM kinase 2 [p Thr505] Antibody.
quantity: 0.05 ml (also 0.025 ml)
price: 349 USD
to the supplier
gene information - rat Limk2
- synonym:Limk2b; Link2
- description:LIM domain kinase 2
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Limk2 antibody
linkout
questions and comments