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- domestic goat polyclonal
reactivity: mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 3
Detection of Galectin-9 antibody in Mouse Thymocytes antibody by Flow Cytometry.
quantity: 100 ug (also 25 ug)
price: 479 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (ER1909-31) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse colon tissue lysate (40 µg/Lane) Lane 4: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1909-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Galectin 9 antibody (ER1909-31) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1909-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (PSH02-45)
reactivity: human, rat
application: western blot 
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721822) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 35 kDa Exposure time: 2 minutes 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721822) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH02-45)
reactivity: human, rat
application: western blot 
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA750810) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 35 kDa Exposure time: 2 minutes 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750810) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Lgals9
- description:galectin 9
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product type
- Lgals9 antibody
- Lgals9 protein
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