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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (JE38-88)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of LEFTY1+LEFTY2 on different lysates with Rabbit anti-LEFTY1+LEFTY2 antibody (HA722177) at 1/1,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: SiHa cell lysate (15 µg/Lane) Lane 3: NCCIT cell lysate (15 µg/Lane) Lane 4: HeLa cell lysate (15 µg/Lane) Lane 5: ES-D3 cell lysate (15 µg/Lane) Lane 6: F9 cell lysate (15 µg/Lane) Lane 7: ES-W4 cell lysate (15 µg/Lane) Lane 8: Mouse heart tissue lysate (30 µg/Lane) Lane 9: Mouse lung tissue lysate (30 µg/Lane) Lane 10: Mouse embryo tissue lysate (30 µg/Lane) Lane 11: Rat heart tissue lysate (30 µg/Lane) Lane 12: Rat lung tissue lysate (30 µg/Lane) Lane 13: Rat embryo tissue lysate (30 µg/Lane) Predicted band size: 41 kDa Observed band size: 28-41 kDa Exposure time: 1 minute ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722177) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Lefty1
- synonym:RGD1561867
- description:left right determination factor 1
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product type
- Lefty1 antibody
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