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- rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1 - rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA - rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of LARS using anti-LARS antibody (A01109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates,. Lane 2: human K562 whole cell lysates,. Lane 3: human THP-1 whole cell lysates,. Lane 4: human Caco-2 whole cell lysates,. Lane 5: rat liver tissue lysates,. Lane 6: mouse brain tissue lysates,. Lane 7: mouse liver tissue lysates,. Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARS antigen affinity purified polyclonal antibody (Catalog # A01109) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARS at approximately 134KD. The expected band size for LARS is at 134KD.
quantity: 100 ug/vial
price: 240 USD
to the supplier - rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay 
Raji cells were subjected to SDS PAGE followed by western blot : with LARS antibody at dilution of 1:2000
Immunohistochemical of paraffm-embedded human colonusing LARS antibody at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells., using LARS antibodyat 1 :25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudo color =DAPI (fluorescent DNA dye)
quantity: 0.06 mL
price: 195 USD
to the supplier
gene information - rat Lars
- synonym:leucine--tRNA ligase, cytoplasmic; leucyl-tRNA synthetase, cytoplasmic
- description:leucyl-tRNA synthetase
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product type
- Lars antibody
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