rat LOC682330 antibody | 10 products in Validated Antibody Database; 10 cited in the literature; 25 total from 6 suppliers

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Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade
Abcam
catalog: ab10799

total number of products: 8

mouse monoclonal (mAbcam 10799)
reactivity: human, mouse, rat, common platanna
application: western blot, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 50

quantity: 100 µg
price:
to the supplier
Rabbit polyclonal to Histone H3 (acetyl K18) - ChIP Grade
Abcam
catalog: ab1191

total number of products: 8

rabbit polyclonal
reactivity: common tobacco, human, mouse, rat, fruit fly
modification: acetylated
application: western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, other
citations: 43

quantity:
price:
to the supplier
Rabbit polyclonal to Histone H3 (tri methyl K79) - ChIP Grade
Abcam
catalog: ab2621

total number of products: 8

rabbit polyclonal
reactivity: budding yeasts, fission yeast, human, mouse, rat, fruit fly, Caenorhabditis elegans
application: western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation
citations: 28

quantity:
price:
to the supplier
Histone H3 (mono methyl K4) antibody
Biorbyt
catalog: orb312274

rabbit polyclonal
reactivity: human, mouse, rat
modification: methylated
application: western blot, ELISA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 ug, 200 ug
price: 165, 215 USD
to the supplier
Anti-Histone H3 (mono methyl R17) Rabbit Monoclonal Antibody
Boster
catalog: M12477-4

total number of products: 10

rabbit monoclonal (BDI-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of Histone H3 (mono methyl R17) expression in HeLa cell lysate (M12477-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-4) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A

Immunohistochemical analysis of paraffin-embedded mouse colon, using Histone H3 (mono methyl R17) Antibody(M12477-4). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-4)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 299 USD
to the supplier
Anti-Methyl-Histone H3 (di K4) Rabbit Monoclonal Antibody
Boster
catalog: M12477-1

total number of products: 10

rabbit monoclonal (AFF-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human liver, using Methyl-Histone H3 (di K4) Antibody(M12477-1). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-1)overnight at 4 . Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Methyl-Histone H3 (di K4) expression in HeLa cell lysate (M12477-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier
Anti-Phospho-Histone H3 (S10) Monoclonal Antibody
Boster
catalog: MP12477

total number of products: 10

rabbit monoclonal (BAE-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Figure 2. IHC analysis of HIST1H3A using anti-HIST1H3A antibody (MP12477). HIST1H3A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (MP12477) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of HIST1H3A using anti-HIST1H3A antibody (MP12477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A antigen affinity purified polyclonal antibody (Catalog # MP12477) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HIST1H3A.
quantity: 100 ug
price: 299 USD
to the supplier
Histone H3 monoclonal antibody, clone 1G1
Abnova
catalog: MAB15247

mouse monoclonal (1G1)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price:
to the supplier
Anti-Histone H3 antibody produced in rabbit
MilliporeSigma
catalog: H0164

total number of products: 4

rabbit polyclonal
reactivity: domestic silkworm, human, mouse, rat, chicken, zebrafish , fruit fly
application: western blot, immunohistochemistry
citations: 26

quantity:
price:
to the supplier
Anti-acetyl-Histone H3 (Ac-Lys 9 ) antibody, Mouse monoclonal
MilliporeSigma
catalog: H0913

total number of products: 4

mouse monoclonal (AH3-120)
reactivity: human, mouse, rat, chicken, cow, Caenorhabditis elegans
application: western blot, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 6

quantity:
price:
to the supplier
Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit
MilliporeSigma
catalog: D5567

total number of products: 4

rabbit polyclonal
reactivity: human, mouse, rat, chicken, cow, thale cress, Caenorhabditis elegans
application: western blot, chromatin immunoprecipitation
citations: 4

quantity:
price:
to the supplier
phospho Histone H3 (Thr11) Antibody, clone N123/48
EMD Millipore
catalog: MABE435

mouse monoclonal (N123/48)
reactivity: rat
modification: phosphorylated
application: western blot, immunocytochemistry

quantity: 100 µL
price:
to the supplier