rat LOC498276 antibody | 3 products in Validated Antibody Database; 4 cited in the literature; 10 total from 6 suppliers

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FCGR2A / CD32a
Proteintech Group
catalog: 15625-1-AP

rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry
citations: 3

The CD32 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CD32. This antibody recognizes human antigen. The CD32 antibody has been validated for the following applications: ELISA, WB, FC analysis.
quantity: 150 ul
price: 299 USD
to the supplier
CD16a / FCGR3A Antibody, Rabbit MAb
Sino Biological
catalog: 80017-R041

rabbit monoclonal (041)
reactivity: rat
application: ELISA

quantity: 100µL
price: 199 USD
to the supplier
CD32A Antibody (aa47-285) LS-C296190
LifeSpan Biosciences
catalog: LS-C296190

rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of recombinant FCGR2A / CD32A. This image was taken for the unconjugated form of this product. Other forms have not been tested.

quantity:
price:
to the supplier
CD16 Monoclonal Antibody (ASH 1975)
Invitrogen
catalog: MA1-7633

mouse monoclonal (ASH 1975)
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

quantity: 100µg
price: US 338
to the supplier
CD16 Polyclonal Antibody
Invitrogen
catalog: PA5-87036

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100µL
price: US 439
to the supplier
FCGR2A Polyclonal Antibody
Invitrogen
catalog: PA5-95557

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100µg
price: US 345
to the supplier
Anti-FCGR2A Picoband Antibody
Boster
catalog: A01450-1

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of FCGR2A using anti-FCGR2A antibody (A01450-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. Lane 2: rat brain tissue lysates. Lane 3: mouse Neuro-2a whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR2A antigen affinity purified polyclonal antibody (Catalog # A01450-1) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR2A at approximately 40KD. The expected band size for FCGR2A is at 35KD.

Figure 2. IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1). FCGR2A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1). FCGR2A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 280 USD
to the supplier
CD32
BD Biosciences
catalog: 550271

total number of products: 3

mouse monoclonal (D34-485)
reactivity: rat
application: flow cytometry, blocking or activating experiments
citations: 4

quantity: 0.5 mg
price: 200.00 USD
to the supplier