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- rabbit monoclonal (AC-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
Western blot analysis of Histone H2B (acetyl K20) expression in Hela cell treated with TSA lysate (P12718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H2BB monoclonal antibody (Catalog # P12718) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H2BB
quantity: 100 ug/vial
price: 299 USD
to the supplier - rabbit polyclonal
reactivity: human, mouse, rat, dog, chicken, zebrafish , fruit fly
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - Acetyl-Histone H2B (Lys20) Polyclonal Antibody
Invitrogen
catalog: PA5-17821
total number of products: 4rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - Acetyl-Histone H2B (Lys5) Polyclonal Antibody
Invitrogen
catalog: PA5-17779
total number of products: 4rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
gene information - rat LOC102549061
- synonym:histone H2B type 1-B
- description:histone H2B type 1-N-like
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- LOC102549061 antibody
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