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- rabbit polyclonal
reactivity: human, mouse, rat, sheep
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 1
The GSTM3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GSTM3. This antibody recognizes human, mouse, rat antigen. The GSTM3 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
quantity: 150 ul
price: 299 USD
to the supplier - rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section 
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using GST Mu5 (PA5-28892) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded SG xenograft using GST Mu5 (PA5-28892) antibody at 1:500 dilution.
Western Blot analysis of GSTM5 using anti-GSTM5 Polyclonal Antibody (PA5-28892), in Mouse Testis (50 ug of cell lysate) and at a dilution of 1:10000.
quantity: 100µL
price: US 345
to the supplier - rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot - rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry 
Figure 1. Western blot analysis of GSTM3 using anti-GSTM3 antibody (A03298-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,. Lane 2: rat brain tissue lysate,. Lane 3: mouse testis tissue lysate,. Lane 4: mouse brain tissue lysate,. Lane 5: human Hela cell lysate,. Lane 6: human MCF-7 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM3 antigen affinity purified polyclonal antibody (Catalog # A03298-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM3 at approximately 26KD. The expected band size for GSTM3 is at 26KD.
Figure 2. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1). GSTM3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1). GSTM3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 280 USD
to the supplier
gene information - rat LOC100912430
- description:glutathione S-transferase Mu 5-like
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- LOC100912430 antibody
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