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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 3

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse kidney lysates (lanes 3 and 6): - 1-3. Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:200).4-6. Anti-Kir7.1 (extracellular) Antibody, preincubated with Kir7.1 (extracellular) Blocking Peptide (#BLP-PC125).
Expression of Kir7.1 in rat kidney - Immunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:100). Staining is present in both distal (DT) and proximal (PT) tubules and in the collecting ducts (CD) in the renal cortex. Note that staining is absent both in glomeruli (Gl) and blood vessels (arrow). Hematoxilin is used as the counterstain.
Cell surface detection of Kir7.1 in live intact Jurkat (human T cell leukemia) cell line: - ___Cells + Goat-anti-Rabbit-FITC.___Cells +Anti-Kir7.1 (extracellular)Antibody (#APC-125) (1:20) + Goat-anti-Rabbit-FITC.
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: ATTO 488
application: immunohistochemistry, immunocytochemistry
citations: 2

Expression of Kir7.1 in rat kidney - Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Kir7.1 (extracellular)-ATTO Fluor-488 Antibody (#APC-125-AG), (1:50). Kir7.1 staining (green) is present in convoluted tubules in the renal cortex. Glomeruli (G) are negative. Cell nuclei were visualized with Hoechst 33342 (blue).
Peptide (C)SGTLKEPDSSKTP corresponding to amino acid residues 438-450 of rat Synapsin-2 (AccessionQ63537). Intracellular region G.
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to the supplier - domestic rabbit polyclonal (polyclonal)
reactivity: rat
application: western blot, immunohistochemistry, flow cytometry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of KCNJ13/Kir7.1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500215, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-KCNJ13/Kir7.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500215, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - rat Kir7 1
- synonym:Kir7.1
- description:potassium inwardly-rectifying channel, subfamily J, member 13
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Kir7 1 antibody
- Kir7 1 protein
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