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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Kidins220 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of Kidins220 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-65, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Kidins220 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-65, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4): - 1,2. Anti-Kidins220 Antibody (#APZ-017), (1:200).3,4. Anti-Kidins220 Antibody, preincubated with Kidins220 Blocking Peptide (#BLP-PZ017).
Expression of Kidins220 in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Kidins220 Antibody (#APZ-017), (1:1000), followed by goat-anti-rabbit-AlexaFluor-488. A. Kidins220 staining (green) in hippocampal sections is seen in neurons in the pyramidal layer (Pyr) and stratum oriens (OR) (arrows). B. Staining in cerebellum sections shows labeling (green) in neurons in the Purkinje cell layer (horizontal arrows). Cell nuclei are stained with DAPI (blue).
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gene information - rat Kidins220
- synonym:ARMS
- description:kinase D-interacting substrate 220
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Kidins220 antibody
- Kidins220 protein
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