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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1 - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of IL-22RA1 on different lysates with Rabbit anti-IL-22RA1 antibody (HA500327) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500327) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ICC staining of IL-22RA1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500327, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of IL-22RA1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500327, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
gene information - rat Il22ra1
- synonym:RGD1559655
- description:interleukin 22 receptor subunit alpha 1
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Il22ra1 antibody
- Il22ra1 protein
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