Your Filters
product
- domestic rabbit polyclonal (SJ19-05)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution. Lane 1: Rat spleen tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Rat thymus tissue lysate Lysates/proteins at 10 µg per lane. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 59 kDa Observed band size: 80 kDa (Glycosylation)
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse thymus tissue lysate Lane 3: Daudi cell lysate Lysates/proteins at 10 µg per lane. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 59 kDa Observed band size: 80 kDa (Glycosylation)
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-21R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500290, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
gene information - rat Il21r
- description:interleukin 21 receptor
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Il21r antibody
- Il21r protein
questions and comments