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- hamsters monoclonal (MOB47)
reactivity: mouse, rat
application: western blot, neutralization, flow cytometry
citations: 1
IFN-? Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-? R2 Antibody.
quantity: 500 ug (also 25 ug)
price: 549 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500246) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: F9 cell lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Lane 7: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500246) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of IFNGR2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500246, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-IFNGR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IFNGR2 on human skeletal muscle tissue lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of SH-SY5Y cells labeling IFNGR2 with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Ifngr2
- description:interferon gamma receptor 2
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product type
- Ifngr2 antibody
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