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- domestic rabbit monoclonal (PSH03-56)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (HA722015) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: HeLa cell lysate (20 µg/Lane) Lane 8: SH-SY5Y cell lysate (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: A431 cell lysate (20 µg/Lane) Lane 11: Mouse pancreas tissue lysate (40 µg/Lane) Lane 12: Rat kidney tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722015) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH03-56)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (HA750890) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: HeLa cell lysate (20 µg/Lane) Lane 8: SH-SY5Y cell lysate (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: A431 cell lysate (20 µg/Lane) Lane 11: Mouse pancreas tissue lysate (40 µg/Lane) Lane 12: Rat kidney tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750890) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier - unidentified polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of MDA5 expression in HepG2 (A), THP1 (B) whole cell lysates.
Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MDA5 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price:
to the supplier
gene information - rat Ifih1
- synonym:RGD1560496
- description:interferon induced with helicase C domain 1
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product type
- Ifih1 antibody
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