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- mouse monoclonal (6E3-2C3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6E3-2C3 (SMC-232). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody (SMC-232) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal (JJ08-97)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1 - mouse monoclonal (6E3-2C3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry - domestic rabbit monoclonal (JG39-11)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of ORP150 on SH-SY5Y cell lysates with Rabbit anti-ORP150 antibody (ET7108-84) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 111 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Hyou1
- synonym:Cab140; Orp150
- description:hypoxia up-regulated 1
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product type
- Hyou1 antibody
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