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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1
Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA, immunohistochemistry, immunoprecipitation 
Immunofluorescence Microscopy of rabbit anti-Cpn10 antibody. Tissue: Mouse backskin sections of Transgenic mice. Fixation: N/A. Primary antibody: Cpn10 for 1h at RT. Seondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Mitochondrion matrix. Staining: Cpn10 as precipiated green signal.
quantity: 100 µg
price:
to the supplier - domestic rabbit monoclonal (JG82-34)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of Cpn10 on different lysates with Rabbit anti-Cpn10 antibody (ET7108-67) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-67) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cpn10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section 
Western Blot: HSP10/EPF Antibody [NB110-11473] - analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
quantity: 0.05 mg (also 0.025 mg)
price: 279 USD
to the supplier - domestic rabbit monoclonal (0H0X6)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - rat Hspe1
- synonym:Cpn10
- description:heat shock protein family E (Hsp10) member 1
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product type
- Hspe1 antibody
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