Your Filters
product
- domestic rabbit monoclonal (JF0981)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
citations: 6 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Western blot analysis of extracts of variouscell lines, using HMGCR antibody.
quantity: 0.1 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JF0981)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody (ET1702-41) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with ET1702-41 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1702-41 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of ET1702-41 in HeLa cell lysate Lane 3: ET1702-41 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody (ET1702-41) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-41) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JF0981)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody (HA750345) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750345) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with HA750345 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750345 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of HA750345 in HeLa cell lysate Lane 3: HA750345 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody (HA750345) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750345) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - rat Hmgcr
- synonym:3H3M
- description:3-hydroxy-3-methylglutaryl-CoA reductase
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Hmgcr antibody
- Hmgcr inhibitor/activator/substrate/reporter
questions and comments