rat Hist4h4 antibody | 2 products in Validated Antibody Database; 6 cited in the literature; 41 total from 8 suppliers

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Histone H4 [p Ser1] Antibody
Novus Biologicals
catalog: NB21-2000

total number of products: 14

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, chromatin immunoprecipitation, dot blot
citations: 3

Chromatin Immunoprecipitation: Histone H4 [p Ser1] Antibody [NB21-2000] - 2 ug of NB21-2000 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Immunocytochemistry/Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in in nonmitotic, prophase, and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

Immunocytochemistry/Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in nonmitotic and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red)
quantity: 0.05 mg (also 0.025 mg)
price: 299 USD
to the supplier
Histone H4 [Monomethyl Arg3] Antibody
Novus Biologicals
catalog: NB21-2011

total number of products: 14

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, chromatin immunoprecipitation, dot blot
citations: 2

Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - WB analysis of Histone H4 R3me1 in NIH/3T3 histone preps.

Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).

quantity: 0.05 mg (also 0.025 mg)
price: 299 USD
to the supplier
Histone H4 [ac Lys5] Antibody
Novus Biologicals
catalog: NB21-2024

total number of products: 14

rabbit polyclonal
reactivity: human, mouse, rat, fruit fly
application: western blot, immunocytochemistry, chromatin immunoprecipitation, dot blot
citations: 1

Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - WB analysis of H4K5ac in HeLa histone prep.

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - WB analysis of H4K5ac in NIH 3t3 histone prep.
quantity: 0.05 mg (also 0.025 mg)
price: 299 USD
to the supplier
Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]
Abcam
catalog: ab177790

total number of products: 4

rabbit monoclonal (EPR16606)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2

quantity: 10 µl, 100 µl
price:
to the supplier
Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)]
Abcam
catalog: ab201075

total number of products: 4

rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 10 µl, 100 µl
price:
to the supplier
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [EPR18184]
Abcam
catalog: ab177309

total number of products: 4

rabbit monoclonal (EPR18184)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section, dot blot

quantity: 10 µl, 100 µl
price:
to the supplier
Histone H4 (DiMethyl K79) Antibody
Abbexa
catalog: abx134221

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 30 µl
price: 188.5 USD
to the supplier
Histone H4 (MonoMethyl K79) Antibody
Abbexa
catalog: abx134222

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 30 µl
price: 188.5 USD
to the supplier
Anti-Histone H4 (acetyl K16) Monoclonal Antibody
Boster
catalog: M14495-3

total number of products: 5

rabbit monoclonal (COC-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A antigen affinity purified polyclonal antibody (Catalog # M14495-3) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HIST1H4A.

Figure 2. IHC analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-3). HIST1H4A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495-3) overnight at 4Â°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Immunofluorescent analysis of HeLa cells treated with TSA, using Histone H4 (acetyl K16) Antibody .
quantity: 100 ug
price: 299 USD
to the supplier
Anti-Histone H4 (tri methyl K20) Rabbit Monoclonal Antibody
Boster
catalog: M14495

total number of products: 5

rabbit monoclonal (DHB-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Histone H4 (tri methyl K20) expression in HeLa cell lysate (M14495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody (Catalog # M14495) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H4A

Immunohistochemical analysis of paraffin-embedded human stomach, using Histone H4 (tri methyl K20) Antibody(M14495). HIST1H4A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 299 USD
to the supplier
Anti-Histone H4 (acetyl K5) Monoclonal Antibody
Boster
catalog: M14495-4

total number of products: 5

rabbit monoclonal (CCA-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Figure 2. IHC analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-4). HIST1H4A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495-4) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A antigen affinity purified polyclonal antibody (Catalog # M14495-4) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HIST1H4A.
quantity: 100 ug
price: 299 USD
to the supplier
Acetyl Histone H4 antibody
Biorbyt
catalog: orb2562

total number of products: 4

rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunocytochemistry, immunohistochemistry - paraffin section

Immunohistochemical analysis of formalin-fixed and paraffin embedded rat brain tissue (Dilution at:1:200) using Acetyl Histone H4 antibody

IHC-P of mouse intestine tissue (Dilution at:1:200) using Acetyl Histone H4 antibody

IHC-P of mouse intestine tissue (Dilution at:1:200) using Acetyl Histone H4 antibody
quantity: 100 ug, 200 ug
price: 215.0, 350.0 USD
to the supplier
HIST1H4A antibody
Biorbyt
catalog: orb393073

total number of products: 4

rabbit polyclonal
reactivity: human, mouse, rat, cow
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of MCF7 cells using HIST1H4A antibody

Immunohistochemical staining of human lung cancer using HIST1H4A antibody

Western blot analysis of HeLa (Lane 1), PC3 (Lane 2), MCF7 (Lane 3), NIH3T3 (Lane 5) whole cell lysates using HIST1H4A antibody
quantity: 30 ul (Trial size), 100 ul, 200 ul
price: 106.0, 169.0, 243.0 USD
to the supplier
HIST4H4 antibody
Biorbyt
catalog: orb49024

total number of products: 4

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of extracts of HeLa cell line using HIST4H4 antibody

Immunohistochemical staining of rat brain tissue using HIST4H4 antibody (dilution of 1:200)
quantity: 50 ul, 100 ul, 200 ul
price: 170.0, 230.0, 360.0 USD
to the supplier
Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Polyclonal Antibody
Epigentek
catalog: A-3708-100

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 µg
price:
to the supplier
Histone H4R3 Monomethyl (H4R3me1) Polyclonal Antibody
Epigentek
catalog: A-3717-100

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

quantity: 100 µg
price:
to the supplier
Histone H4R3 Dimethyl Symmetric (H4R3me2s) Polyclonal Antibody
Epigentek
catalog: A-3718-100

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 µg
price:
to the supplier
Histone H4 pS1 Antibody
Rockland Immunochemicals
catalog: 600-401-I96

total number of products: 7

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Immunofluorescence of Rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

Western Blot of Rabbit Anti-Histone H4 [p Ser1] Antibody. Lane 1: HeLa histone prep. Load: 30 µg per lane. Primary antibody: Histone H4 [p Ser1] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Immunofluorescence of Rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
quantity: 50 µg
price: 401 USD
to the supplier
Histone H4 K5ac Antibody
Rockland Immunochemicals
catalog: 600-401-I98

total number of products: 7

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H4 [ac Lys5] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of Histone H4 K5ac was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
quantity: 50 µg
price: 401 USD
to the supplier
Histone H4 K8ac Antibody
Rockland Immunochemicals
catalog: 600-401-I99

total number of products: 7

rabbit polyclonal
reactivity: human, mouse, rat, Caenorhabditis elegans
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H4 [ac Lys8] Antibody. Lane 1: HeLa histone prep. Lane 2: 3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys8] at 0.2 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~11 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H4 [ac Lys8] Antibody. Lane 1: K8 Ac. Lane 2: K8 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 [ac Lys8] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Chromatin Immunoprecipitation Rabbit Anti-Histone H4 [ac Lys8] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of Histone H4 K8ac and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price: 401 USD
to the supplier
Monoclonal Anti-triMethyl-Histone H4 (triMe-Lys20) antibody produced in mouse
MilliporeSigma
catalog: SAB4200253

mouse monoclonal (6F8-D9)
reactivity: human, mouse, rat
application: western blot

quantity:
price:
to the supplier
MonoclonalAnti-methyl-Histone H4 (Me-Lys20) antibody produced in mouse
MilliporeSigma
catalog: SAB4200252

mouse monoclonal (5E10-D8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity:
price:
to the supplier