rat Hist1h3a antibody | 60 (1 knockout-validated) products in Validated Antibody Database; 72 cited in the literature; 140 total from 4 suppliers

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Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade
Abcam
catalog: ab1012

total number of products: 7

mouse monoclonal (mAbcam1012)
reactivity: hamsters, budding yeasts, human, mouse, rat, cow, zebrafish , common platanna, thale cress
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, ChIP-Seq
citations: 102

quantity: 100 ug
price:
to the supplier
Histone H3 (mono methyl K9) antibody - ChIP Grade
Abcam
catalog: ab9045

total number of products: 7

rabbit polyclonal
reactivity: African green monkey, human, mouse, rat, cow, fruit fly, thale cress, Caenorhabditis elegans
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 45

quantity: 100 ug
price:
to the supplier
Rabbit polyclonal to Histone H3 (acetyl K18) - ChIP Grade
Abcam
catalog: ab1191

total number of products: 7

rabbit polyclonal
reactivity: common tobacco, human, mouse, rat, fruit fly
modification: acetylated
application: western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, other
citations: 43

quantity:
price:
to the supplier
Phospho-Histone H3 (Ser10) Polyclonal Antibody
Invitrogen
catalog: PA5-17869

total number of products: 72

rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288c, human, mouse, rat, fruit fly, common platanna
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 4

Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
quantity: 100µL
price: US 345
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-16183

total number of products: 72

rabbit polyclonal
reactivity: Chlamydomonas reinhardtii, human, mouse, rat, chicken, cow, zebrafish , fruit fly
application: western blot, immunocytochemistry
citations: 4

quantity: 100µL
price: US 345
to the supplier
Histone H3 Monoclonal Antibody (E.960.2)
Invitrogen
catalog: MA5-15150

total number of products: 72

rabbit monoclonal (E.960.2)
reactivity: Chinese hamster, human, mouse, rat, dog, cow, pig, zebrafish , fruit fly
application: western blot
citations: 3

quantity: 100µL
price: US 345
to the supplier
Histone H3 Antibody
Cell Signaling Technology
catalog: 9715

total number of products: 57

rabbit polyclonal
reactivity: human, mouse, rat, cow, fruit fly, common platanna
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 144

quantity:
price:
to the supplier
Histone H3-pThr11
Neuromab
catalog: 73-223

total number of products: 4

mouse monoclonal (N123/19)
reactivity: rat
citations: 1

quantity: 5 ml
price: 269 USD
to the supplier