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- rabbit monoclonal (SN72-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Immunocytochemistry/Immunofluorescence: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Staining Histone H2B(acetyl K20) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100 ul
price: 279 USD
to the supplier - rabbit monoclonal (HAD-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation 
Western blot analysis of Histone H2B expression in A431 cell lysate (M11068). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H2BK monoclonal antibody (Catalog # M11068) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H2BK
quantity: 100 ug/vial
price: 299 USD
to the supplier - rabbit polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot - mouse monoclonal (CL5101)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - mouse monoclonal (CL5048)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
gene information - rat Hist1h2bk
- synonym:Hist1h2bc; histone H2B; histone cluster 1, H2bc; histone cluster 1, H2bk
- description:histone cluster 1 H2B family member k
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- Hist1h2bk antibody
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