rat H3f4 antibody | 16 products in Validated Antibody Database; 32 cited in the literature; 153 total from 4 suppliers

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Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade
Abcam
catalog: ab1220

total number of products: 5

mouse monoclonal (mAbcam 1220)
reactivity: fission yeast, African green monkey, human, mouse, rat, zebrafish , fruit fly , thale cress, roundworm
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, ChIP-Seq
citations: 431

quantity: 100 µg
price:
to the supplier
Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade
Abcam
catalog: ab8580

total number of products: 5

domestic rabbit polyclonal
reactivity: fission yeast, human, mouse, rat, fruit fly , thale cress, roundworm
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, other
citations: 318

quantity: 10 µg
price:
to the supplier
Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade
Abcam
catalog: ab9050

total number of products: 5

domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, brewer's yeast, fruit fly , Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq
citations: 137

quantity: 100 µg
price:
to the supplier
Phospho-Histone H3 (Ser10) Polyclonal Antibody
Invitrogen
catalog: PA5-17869

total number of products: 145

domestic rabbit polyclonal
reactivity: human, mouse, rat, fruit fly , Xenopus laevis
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 29

Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
quantity: 100 uL
price: US 514.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-16183

total number of products: 145

domestic rabbit polyclonal
reactivity: Chlamydomonas reinhardtii, human, mouse, rat
application: western blot, immunocytochemistry
citations: 22

quantity: 100 uL
price: US 514.00
to the supplier
H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified
Invitrogen
catalog: MA5-11198

total number of products: 145

domestic rabbit monoclonal (G.299.10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, other
citations: 12

quantity: 100 uL
price: US 524.00
to the supplier
p-Ac-Histone H3 (APH3-64)
Santa Cruz Biotechnology
catalog: sc-56739

mouse monoclonal (APH3-64)
reactivity: rat
application: western blot, immunoprecipitation

quantity: 100 µg/ml
price: 316 USD
to the supplier
Anti-Histone H3 antibody produced in rabbit
MilliporeSigma
catalog: H0164

total number of products: 2

domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot
citations: 52

quantity:
price:
to the supplier