TFIIE alpha
HUABIO
catalog: HA721376

domestic rabbit monoclonal (JE66-41)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of TFIIE alpha on different lysates with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: Daudi cell lysate (15 µg/Lane) Lane 4: HeLa cell lysate (15 µg/Lane) Lane 5: A431 cell lysate (15 µg/Lane) Lane 6: A549 cell lysate (15 µg/Lane) Lane 7: HepG2 cell lysate (15 µg/Lane) Lane 8: HEK-293 cell lysate (15 µg/Lane) Lane 9: NIH/3T3 cell lysate (15 µg/Lane) Lane 10: MCF7 cell lysate (15 µg/Lane) Lane 11: Mouse testis tissue lysate (30 µg/Lane) Lane 12: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 49.5 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721376) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721376) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721376) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier