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- Glutathione Synthetase Antibody (OTI1B8)
Novus Biologicals
catalog: NBP2-03351
total number of products: 4mouse monoclonal (OTI1B8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 3 - Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free
Novus Biologicals
catalog: NBP2-70854
total number of products: 4mouse monoclonal (OTI1B8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
quantity: 100 ug
price: 579 USD
to the supplier - Glutathione Synthetase Antibody (JB95-33)
Novus Biologicals
catalog: NBP2-75513
total number of products: 4domestic rabbit monoclonal (JB95-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513]
quantity: 100 ul
price: 449 USD
to the supplier - domestic rabbit monoclonal (JB95-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Glutathione Synthetase on different lysates with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Glutathione Synthetase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-62) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Glutathione Synthetase on different lysates with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Human liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Glutathione Synthetase antibody (ET7107-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - rat Gss
- description:glutathione synthetase
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Gss antibody
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