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- mouse monoclonal (A5E6)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA601019) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse lung tissue lysate Lane 5: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 28 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601019) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA601019) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601019) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA601019) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601019) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier - mouse monoclonal (A5E6-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 28 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601262) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier - mouse monoclonal (A5E6-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA610137) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 28 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610137) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA610137) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610137) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA610137) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610137) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier
gene information - rat Gp9
- description:glycoprotein IX (platelet)
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- Gp9 antibody
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