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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 12 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 5 - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry
citations: 4
Western blot using Rockland's affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
quantity: 100
price: 509.00 USD
to the supplier - domestic rabbit monoclonal (PSH11-23)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of Gli2 on different lysates with Rabbit anti-Gli2 antibody (HA751402) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: F9 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Rat pancreas tissue lysate (40 µg/Lane) Lane 6: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 168 kDa Observed band size: 133 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751402) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (PSH11-23)
reactivity: human, mouse, rat
application: western blot
gene information - rat Gli2
- description:GLI family zinc finger 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Gli2 antibody
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