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- domestic rabbit monoclonal (JE55-12)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Glycerol Kinase on different lysates with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Glycerol Kinase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61/57 kDa Observed band size: 61/57 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-96) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Glycerol Kinase on different lysates with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/500 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61/57 kDa Observed band size: 61/57 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Glycerol Kinase on different lysates with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/500 dilution. Lane 1: Mouse bone marrow tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61/57 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Gk
- synonym:ASTP; Gyk
- description:glycerol kinase
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- Gk antibody
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