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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1,2. Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:200).3,4. Anti-Connexin-45 (GJC1) Antibody, preincubated with Connexin-45/GJC1 Blocking Peptide (#BLP-CC207).
Expression of Connexin-45 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:400), followed by anti-rabbit-Cy3 antibody. Cx45 staining (red) appears in the neuropil of layers stratum oriens and stratum radiatum but not in the pyramidal layer. Nuclei are stained with DAPI (blue).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: - 1.Anti-Connexin-45 (GJC1) Antibody (#ACC-207) (1:200).2. Anti-Connexin-45 (GJC1) Antibody preincubated with the negative control antigen.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay - mouse monoclonal
reactivity: human, rat
application: immunocytochemistry, flow cytometry - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Connexin 45 / GJA7 / Cx45 on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: Anti-Connexin-45 Antibody; Lane 2: Anti-Connexin-45 Antibody, pre-incubated with the immunizaiton peptide.
ICC staining Connexin 45 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ICC staining Connexin 45 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Gjc1
- synonym:Cx45; Cxnq; Gja7
- description:gap junction protein, gamma 1
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product type
- Gjc1 antibody
- Gjc1 protein
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