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- mouse monoclonal (2H3)
reactivity: human, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of ASNA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500051, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: 293T cell lysate
Immunocytochemistry analysis of 293T cells labeling ASNA1 with Rabbit anti-ASNA1 antibody (HA500051) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ASNA1 antibody (HA500051) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of 293T cells labeling ASNA1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500051, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
gene information - rat Get3
- synonym:Asna1
- description:guided entry of tail-anchored proteins factor 3, ATPase
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Get3 antibody
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